Furthermore, the logistic regression analysis reaffirmed the effects of HLA alleles, female sex, and lower age at onset on ANA positivity, with DRB1*08, DRB1*11, and female sex having a larger effect than age at onset, suggesting that these factors collectively contribute to the complex autoimmunity in ANA positive JIA. This evidence concerns the gene HLA-DRB1 and juvenile idiopathic arthritis.