To further explore the impact of the Xianhecao-Huanglian drug combination on autophagy in DSS-induced IBD mice, we examined the expression of proteins associated with autophagy, including ATG12, Beclin-1, LC3A/B, and p62 (Fig. 6A-F), in colitis tissues using Western blot analysis. This evidence concerns the gene ATG12 and inflammatory bowel disease.