CD8A and colorectal cancer: In addition, since TLS-associated regulatory T cells (Tregs) have been found to attenuate the positive prognostic effect of TLS-associated CD8+ T cells in human non-small cell lung cancer, colorectal cancer, and soft tissue sarcoma [44, 53, 54], we assessed the presence of putative Tregs (CD3+FOXP3+) in LAs in the same subset of biopsies (n = 2).