Sanger sequencing analysis revealed that the astrocytomaused inthe human astrocytoma model harbors two heterozygous mutations inTP53: p.W146* (c.437G > A), which leads to a loss of p53 proteinfunction,and p.R280K, which results in increased cell migration and invasionand increased cell survival.17,18 This is a reason forthe increase in the astrocytoma’s degree up to grade 4. Here, TP53 is linked to astrocytoma (excluding glioblastoma).