T cells expressing a CAR containing a distal IL7Rα domain (CD28.IL7R.ζ) had superior leukemia control in vivo when compared with the T cells expressing a CAR with a proximal IL7Rα domain (IL7R.CD28.ζ) and with CAR-T cells secreting IL7 (Fig. 7B–D). The gene discussed is CD28; the disease is leukemia.