In an animal model induced with DEN and 2-acetylaminofluorene (2-AAF), kaempferol inhibited rat hepatocyte activity, increased apoptotic cell proportion, caspase 3 enzyme activity, ROS production, and cytochrome C release into the cytoplasm, suggesting its role in enhancing ROS production and regulating oxidative stress to induce apoptosis in HCC cells. This evidence concerns the gene CASP3 and hepatocellular carcinoma.