In contrast, the majority of tetramer negative (Tetramer−) CD4+ T cells isolated from B16-3340 and B16-EV tumors, irrespective of SFB colonization, were regulatory-like T cells, expressing both T-bet and Foxp3 (Extended Data Fig. 2h, i), a phenotype associated with strong suppression of anti-tumor immune responses31. This evidence concerns the gene CD4 and neoplasm.