NLGN4X and colitis: We validated the effect of the translated protein Nlgn173 on colitis development using various expression constructs, including circNlgn, linNlgn (expressing a linear mRNA to translate Nlgn173), Precursor (expressing a linear RNA that could not translate Nlgn173), circ-mut (expressing a mutated circRNA that could not translate Nlgn173), circ-164 (expressing a mutated circRNA that could translate a smaller protein, Nlgn164), the full-length Nlgn, and the control vector, as previously described [19].