Downregulation of PCK1 protein expression was observed in both NAFLD patients and animal models of mice fed a high-fat diet and deficiency of the glycoheterotrophic enzyme PCK1 may promote NAFLD in male mice via the PI3K/AKT/PDGF axis (Ye et al., 2023). The gene discussed is AKT1; the disease is metabolic dysfunction-associated steatotic liver disease.