The first principal component (PC1) of HepIRDS, accounting for a major portion of the variance in HepIRDS gene expression (65%), along with most genes in the HepIRDS, displayed a significant correlation with markers of liver health, including ALT/aspartate aminotransferase ratio, liver percentage of body weight, liver weight, liver steatosis, and plasma cholesterol levels (Fig. 4B and Fig. S3A). The gene discussed is GPT; the disease is Hepatic steatosis.