The first principal component (PC1) of HepIRDS, accounting for a major portion of the variance in HepIRDS gene expression (65%), along with most genes in the HepIRDS, displayed a significant correlation with markers of liver health, including ALT/aspartate aminotransferase ratio, liver percentage of body weight, liver weight, liver steatosis, and plasma cholesterol levels (Fig. 4B and Fig. S3A). This evidence concerns the gene GPT and fatty liver disease.