As IgM antibodies can persist years after infection, and could be elevated with reinfection with a different strain, and because the IgM can give false positive results and thus have a limited use in determining incidence, severity and timing of T. gondii infection (Dhakal et al. 2015), we have not integrated analysis of IgM seropositivity and serointensity into our analysis of IgG serointensity. The gene discussed is CD40LG; the disease is infection.