ROC curve analysis for relative gene expression levels of both IL-1β and NLRP3 to discriminate between early (S0, S1, S1-2) and late (S2 + S2-3 + S3) post-MAFLD hepatic steatosis showed that IL-1β at cutoff value > 33.98 had an AUC of 0.656, sensitivity of 42.7%, specificity of 93.55%, PPV of 95%, and NPV of 36.2% and accuracy of 68.1. The gene discussed is IL1B; the disease is fatty liver disease.