Mechanistically, the O-GlcNAcylation of PGK1 at Thr-255 not only increases PGK1 kinase activity to increase lactate production but also triggers the translocation of PGK1 into mitochondria to phosphorylate and inhibit pyruvate dehydrogenase in colon cancer cells. The gene discussed is PGK1; the disease is colonic neoplasm.