After finding that the NEFH promoter and DiATOH7 enhancer are capable of driving eGFP expression in RGCs at levels comparable to those of CMV.hSNCG, we next examined whether these enhancer/promoter combinations can also induce similar expression of hSIRT1, which has previously been shown to exert neuroprotective effects in various optic neuropathy models.9 Here, SIRT1 is linked to optic nerve disorder.