After finding that the NEFH promoter and DiATOH7 enhancer are capable of driving eGFP expression in RGCs at levels comparable to those of CMV.hSNCG, we next examined whether these enhancer/promoter combinations can also induce similar expression of hSIRT1, which has previously been shown to exert neuroprotective effects in various optic neuropathy models.9 The gene discussed is NEFH; the disease is optic nerve disorder.