According to the above informatics, qRT-PCR was used to detect the expression of MMP3, MMP9, TIMP1, VEGFA in CRC cells after luteolin treatment, and found that luteolin might down-regulate the expression of MMP3, MMP9, TIMP1, VEGFA in RKO cell lines, and only the expression of MMP3, MMP9 was decreased in SW480 (Figure 7K, 7L). The gene discussed is TIMP1; the disease is colorectal carcinoma.