Before we started, we detected the expression levels of MMP3, MMP9, TIMP1, VEGFA in various colon cancer cell lines by qRT-PCR assay, and found that these genes were highly expressed in RKO and SW480, and we chose RKO and SW480 for the following experiments (Supplementary Figure 2). The gene discussed is TIMP1; the disease is malignant colon neoplasm.