To this end, CD4+ Th cells were isolated from the spleens of colitis mice treated with cADSCs primed with each condition, and the frequencies of Th1 cells expressing IFN-γ as an intracellular cytokine, Th2 cells expressing IL-4, Th17 cells expressing IL-17A, and T-regulatory (Treg) cells expressing both CD25 on the cell surface and the transcription factor Foxp3 in the nucleus were determined using flow cytometry. This evidence concerns the gene FOXP3 and colitis.