To this end, CD4+ Th cells were isolated from the spleens of colitis mice treated with cADSCs primed with each condition, and the frequencies of Th1 cells expressing IFN-γ as an intracellular cytokine, Th2 cells expressing IL-4, Th17 cells expressing IL-17A, and T-regulatory (Treg) cells expressing both CD25 on the cell surface and the transcription factor Foxp3 in the nucleus were determined using flow cytometry. Here, CD4 is linked to colitis.