Next, we treated NB cells with 1 μM TRA or 10 μM SEL for 8 or 24 h, which resulted in a notable reduction in the mRNA levels of MYCN and key EP-TF risk genes (RUVBL1, TIGD1 and SUPT16H) in both BE (2)-C and SK-N-BE2 cells (Figure 9G). This evidence concerns the gene MYCN and neuroblastoma.