Furthermore, we quantitatively assessed Hsp90α expression in the culture supernatant of Panc-1-gem cells and 4 other PC cell lines (Panc-1, Aspc-1, BxPc-3, and SW1990) using ELISA and observed significantly greater expression in the Panc-1-gem cell supernatant than in the other cell lines. The gene discussed is HSP90AA1; the disease is pachyonychia congenita.