GAGs are long, linear polysaccharidesfound in the ECM of breast tissues which are highly negatively chargedin nature.89−91 They maintain tissue structure, hydration, and signaling.A study compared healthy breast tissues to breast cancer tissues anddisplayed differences associated with GAGs such as enhanced sulfation,changes in chain length (15% increase), elevated quantity (2x), andcomposition.92 Pereira et al. indicatedthat a reduction in Galectin-3 (Gal-3) during breast tumor growthled to modified GAG expression in the cancerous cells (Figure 5). The gene discussed is LGALS3; the disease is breast carcinoma.