TP53 and cancer: To quantify drug-induced readthrough of diverse PTCs, we constructed a library containing 3,498 PTCs that cause Mendelian diseases reported in ClinVar1, 2,372 recurrent somatic PTCs in cancer genes (721 from The Cancer Genome Atlas (TCGA)17 plus 1,651 from MSK-IMPACT18) and a TP53 control no-nonsense variant (n = 5,871; Fig. 1b; Methods).