Diet-induced obesity on a HFD is likely controlled by a variety of genetic factors, while db/db mice, as recognized leptin receptor gene-deficient mice recessive mutations on chromosome 4, were used to establish a diabetic-obese mouse model and to investigate whether exogenous ANXA1 (Ac2-26) has a therapeutic effect on obesity. Here, ANXA1 is linked to obesity due to melanocortin 4 receptor deficiency.