The comparative analysis of the laxative effects of raw and cooked rhubarb in a mouse model of heat-induced constipation revealed that the more potent effect of raw rhubarb might be induced by the stimulation of intestinal smooth muscle contractions, mediated by increased secretion of intestinal acetylcholinesterase (Ach E) and SP and the augmentation of intracellular Ca2+ concentration (Wu et al., 2014). This evidence concerns the gene ACHE and constipation disorder.