We co-expressed Flag-tagged versions of the non-DNA binding mutant proteins MITF-Wh, MITF-mi, and MITF-ew (Table 1) together with the MITF-WT-GFP, MITF-sp-GFP, or MITF-sl-GFP proteins in A375P melanoma cells which express little endogenous MITF (Wouters et al, 2020) followed by co-immunoprecipitation (co-IP) using FLAG-antibodies. Here, MITF is linked to melanoma.