Immunoblotting analysis showed that the accumulation of LC3B‐II, SQSTM1, BCL2‐associated protein (Bax) and cleaved poly‐ADP ribose polymerase (PARP) in the tumor tissues were increased following ALO treatment compared with the CT group (Figure 5G), which suggested that ALO inhibited autophagic flux and induced apoptosis of NSCLC cells in vivo. The gene discussed is SQSTM1; the disease is neoplasm.