To evaluate the functional significance of Tier 2 signalling derived from RTK PRMs we identified overrepresented protein class, biological process and molecular function terms amongst interactors for the three RTK-cell line combinations demonstrating the highest number of interactors: the EGFR C-terminal tail in H520 LSCC cells and U251 GBM cells, and the FGFR2 C-terminal tail in SKBR3 BrAC cells. This evidence concerns the gene FGFR2 and glioblastoma.