Interestingly, co-immunofluorescence staining revealed novel MKK3 roles in promoting AURKA functions, since MKK3 co-localized with AURKA both in the cytoplasmic and nuclear regions, and the MKK3-knockdown affected the AURKA nuclear localization (Fig. 4B), wheras the AT9283 treatments shifted the protein complex in cytoplasmic / perinuclear regions (Fig. 4B) without altering the protein–protein interaction in tested CRC lines (Fig. 4A). The gene discussed is MAP2K3; the disease is colorectal carcinoma.