ATG5 and colorectal carcinoma: Furthermore, to test for autophagy in vivo, we treated a CRC mouse model with 2c, 2cNP, and Apt-2cNP and found a time-dependent increase in the RNA expression of autophagy-related genes (Atg5, LC3B, Beclin) and a decrease in p62 expression 24 h, or 48 h after treatment.