It is calculated from the expression of 25 genes, including steroid metabolism genes, cholesterol transporter genes, and their transcriptional regulator SF-1 (steroid factor 1 encoded by NR5A1), which are involved in adrenocortical differentiation and have been shown to affect the prognosis of ACC cases (5, 40). The gene discussed is NR5A1; the disease is adrenal cortex carcinoma.