In this study, we conducted phenotypic studies on both NKG2D (N) CAR-T and IL-15/IL-15Rα-NKG2D (N15) CAR-T cells, and then explored their anti-tumor and antiviral efficacy in vitro and in vivo in comparison with non-transduced (NT)-T cell controls, aiming at investigating a novel potential approach for the treatment of EBV-PTLD patients. Here, IL15 is linked to neoplasm.