Human induced pluripotent stem cell (iPSC)-derived skeletal myocyte (iSkM) cell models of healthy (N-iSkM) and DMD (DMD-iSkM) were characterized and utilized to determine if HMGB1 and VCAM1 are affected in a human DMD isolated skeletal muscle model. The gene discussed is HMGB1; the disease is Duchenne muscular dystrophy.