Furthermore, the challenge of primary neonatal rat cardiomyocytes (NRCMs) with Ang II for 48 h augmented cardiomyocyte hypertrophy (Fig. S1J), resulting in a consistent expression pattern for both hypertrophic markers (Fig. S1K) and REST (Fig. S1L, M) with that observed in TAC heart. This evidence concerns the gene REST and persistent truncus arteriosus.