CALR and Increased total leukocyte count: To further investigate whether TLR2 values were related to Jak2 V617F mutation, leukocytosis, thrombocytosis, or anemia status, 90 patients with JAK2 V617F mutations were compared to 11 CALR mutations (including six Type 1 and five Type 2; six ET and five PMF), along with 21 controls (Figure 4(a)).