In vitro analysis showed that blockade of PVRIG or TIGIT increased the number of IFN-γ-producing NK cells under co-culture of breast cancer cells, and the combination of anti-PVRIG and anti-TIGIT antibodies induced a further increase in IFN-γ-producing NK cells and improved cytotoxicity of NK cells (145). The gene discussed is TIGIT; the disease is breast carcinoma.