After infection with the recombinant lentiviruses over-expressing NSF, the OGD-created impairment of autophagic flux was greatly restored, as reflected by decreased autophagic cargoes of LC3-II (Fig. 6F), insoluble p62 (Fig. 6D) and ubiquitinated (Fig. 6H) proteins, accompanied by increased CTSD (Fig. 6G). This evidence concerns the gene CTSD and infection.