To explore the effects of TLR9 on wound healing and HF regeneration, we dropwise added the TLR9 agonist ODN 2395 (4 μg per injection, InvivoGen, America) or the TLR9 antagonist ODN 2088 (4 μg per injection, InvivoGen, America) and TLR9−/− mice (C57BL/6Smoc-Tlr9em1Smoc) (Cat. The gene discussed is TLR9; the disease is hydrops fetalis.