We discovered that HS2ST1 expression correlated with poor prognosis in the TCGA-Liver Hepatocellular Carcinoma (LIHC) cohort and was prognostically relevant in two independent International Cancer Genome Consortium (ICGC) cohorts of hepatocellular carcinoma (HCC) patients. This evidence concerns the gene HS2ST1 and hepatocellular carcinoma.