We next treated multiple IDH1 MT glioma cell lines, HK252, HK213, BT142, & MGG119 (4) and IDH1 WT glioma cell lines, HK157, HK408, HK206, HK350, & HK393 (5) with 1 mM VPA for 48 h and conducted bulk RNA sequencing to get a better understanding of the transcriptomic effect of VPA. This evidence concerns the gene IDH1 and glioma.