Background: Overweight and obesity are escalating public health concerns globally, and India is also experiencing a significant rise in the population of overweight and obese people. Tumour necrosis factor alpha (TNF-α) is a type of cytokine that has garnered attention in particularly obesity-related metabolic disturbances. This study aimed to assess TNF-α levels in overweight and obese adults and explore its correlation with metabolic syndrome and associated biochemical parameters in the northeastern Indian population. This evidence concerns the gene TNF and Obesity.