To explore the intricate mechanisms of JMML pathogenesis, we conducted acomprehensive single-cell RNA sequencing (scRNA-seq) analysis on bone marrow (BM) cellsisolated from mice with induced JMML(Ptpn11E76K/+/Mx1-Cre)6 and wild-type (WT,Ptpn11+/+/Mx1-Cre)control littermates. This evidence concerns the gene MX1 and juvenile myelomonocytic leukemia.