We hypothesized that EGLN1 knockout would lead to HIF-1α stabilization selectively in tRCC cells, given that VHL is retained in this RCC subtype and because 14q deletion (where the HIF1A gene is located) is uncommon in tRCC, in contrast to ccRCC42,82. Here, HIF1A is linked to renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.