As HIF-1α functions a metabolic switch to reprogram metabolism away from OXPHOS and toward glycolysis, we reasoned that EGLN1 knockout could stabilize HIF-1α and thereby have a detrimental effect on tRCC cells, which we have shown to be uniquely dependent on OXPHOS83,84. This evidence concerns the gene HIF1A and renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.