We investigated the cancer Dependency Map (DepMap) portal and database and found that CNS and brain tumor cell lines with high expression of the exon 4 included transcript of CLK1 (≥ third quantile mRNA expression of ENST00000321356) have significantly higher CRISPR dependency (lower scores) compared to CLK1 low expressing cell lines (≤ first quantile) (Wilcoxon p = 0.034, Figure 4A). Here, CLK1 is linked to cancer.