TNF and cancer: To further investigate the impact on DNA repair and other pathways, we performed gene-set enrichment analyses of DNA repair and cancer signaling pathways on these DS oncogenes and TSGs and found that depletion of CLK1 leads to upregulation of TNFA, PI3K/AKT/MTOR, IL6/JAK/STAT3, and apoptosis pathway expression and downregulation of multiple DNA repair pathways (Figure S4C–F).