Through in vitro pulldown tests using purified recombinant proteins, it was shown that ZDHHC6 directly interacts with PPARγ, as depicted in Fig. 5D. Given significant potential correlation of ZDHHC6 with PPARγ, we next integrated the ZDHHC6-interacting proteins obtained from the IP-MS experiment and the upregulated proteins revealed by proteomics analysis, and we identified PPARγ as the protein that interacts with and is regulated by ZDHHC6 using human HCT116, Caco-2, SNU-C2A and HT-29 colon cancer cell lines (Fig. 5E). This evidence concerns the gene ZDHHC6 and colonic neoplasm.