The ZDHHC6 protein expression in human CRC cell lines was significantly decreased in a dose-dependent manner when exposed to a concentration gradient of 2-BP, as shown in Fig. 2F and G. In addition, the administration of 2-BP leads to the inhibition of ZDHHC6, which is directly associated with the decrease in Ki67-positive colon cancer cells. This evidence concerns the gene MKI67 and malignant colon neoplasm.