Biomarker studies from pre- vs. post-tPA comparisons were mostly collected at baseline and at 24 h post-tPA treatment, among which MMP-9 (Inzitari et al, 2013), C-reactive protein (CRP) (Karlinski et al, 2014) and IL-10 (Gori et al, 2017) were related to HT prediction. The gene discussed is IL10; the disease is hematocrit.