The doubling time of HCC-LM3 and SNU-449 cells with HDAC2 overexpression was shortened by 45.6 h and 31.2 h, and the number of colony clusters was 1.8 and 1.9 times higher than controls (Fig. 3C, D and Fig. S2C, D). This evidence concerns the gene HDAC2 and hepatocellular carcinoma.