HDAC2 and hepatocellular carcinoma: We began by examining HDAC2 expression in seven human HCC cell lines (Fig. S1A–C) and then established HDAC2-silenced HepG2 and Huh7 cells, along with HDAC2-overexpressing HCC-LM3 and SNU-449 cells, demonstrating knockdown efficiencies greater than 70% and overexpression efficiencies exceeding three-fold (Fig. S1D–G).