We performed Western blots on control, DIO, Nox2-KO, and DIO Nox2-KO mice and showed that knockout of Nox2 reversed the obesity-induced increase in the protein-level expression of PKC-α and PKC-δ isoforms (Supplemental Figure 3, G and H). The gene discussed is PRKCD; the disease is obesity due to melanocortin 4 receptor deficiency.