To identify the potential pathways involved in obesity-mediated AF, we performed proteomic profiling of pooled LA and RA protein lysates from control, DIO, and DIO Nox2-KO mice using a Q Exactive HF mass spectrometer coupled with an UltiMate 3000 RSLC nanosystem with a Nanospray Flex Ion Source. The gene discussed is CYBB; the disease is obesity due to melanocortin 4 receptor deficiency.