To investigate the role of RGS10 in breast cancer cells, we silenced RGS10 in SKBR3 cells using two independent shRNA constructs, shRNA-RGS10-161 and shRNA-RGS10-506, which had significantly improved transfection efficiency compared to shRNA-RGS10-321 (Figure 2B). This evidence concerns the gene RGS10 and breast cancer.