By observing the growth, development, and motor behavior of zebrafish, and analyzing the mRNA expression of dopamine signaling pathway-associated genes, Parkinson’s disease (PD)-associated genes, and apoptosis-associated genes after ATP1A3 mutation, we further identified the function of the ATP1A3 p.Ala275Pro mutant and explored the influence of this mutant on the zebrafish nervous system. Here, ATP1A3 is linked to Parkinson disease.