Myocardial infarction (MI) is a severe cardiovascular event that can lead to death.Cardiac troponin I (cTnI) is an MI biomarker in the circulation system; however, methodsfor detecting cTnI protein require substantial time, tedious operations, an expensivereader for translating signals, and a lot of reagents. The gene discussed is TNNI3; the disease is myocardial infarction.