SPP1 and neoplasm: This study uncovers the profound impact of anoikis-mediated changes in the tumor microenvironment (TME) at the single-cell level, finding that BRMS1 promotes M2 polarization in microglia and activates the PI3K/AKT signaling pathway through SPP1/CD44-mediated cell interactions, inhibiting GBM cell apoptosis and promoting proliferation, migration, and invasion, leading to adverse effects on the prognosis and immune therapy response of GBM patients.