Intriguingly, the T532A mutation (532Threonine is mutated to 532Alanine) and the GPR50 truncation (amino acid 502-505 is deleted, Δ502-505), which are genetically associated with ASD, bipolar disorder, and depression [11–15], also showed an attenuated binding capability to LC3 in both the duolink assay (Fig. 2G, H) and the Co-IP (Fig. 2I, J; Supplementary Fig. 4). This evidence concerns the gene GPR50 and major depressive disorder.